Journal: Lab on a Chip

Article Title: Functionality integration in stereolithography 3D printed microfluidics using a “print-pause-print” strategy †

doi: 10.1039/d4lc00147h

Figure Lengend Snippet: Microfabricated filters. a) Pictures of microfabricated filters exhibiting the porous circular membrane in the centre and 12 electrical contact pads. b) SEM micrograph of the porous circular membrane supporting sensing electrodes and surrounded by fluidic slits formed by the silicon supporting structure. c) Tomographic cross section showing the subsequent integration of a glass coverslip and a microfabricated filter within a 3D printed fluidic channel. d) Pictures of the 3D printed chip. e) Fluorescence microscopy during the capture of PC3 GFP cells in PBS. f) Bright field image of trapped cells on the membrane at day 0 and day 2, after media renewal.

Article Snippet: Bacteria were imaged using differential interference contrast (DIC) and GFP fluorescence with a specific filter (GFP-3035D, Nikon) and a 40× objective (MRD00405, Nikon).

Techniques: Membrane, Fluorescence, Microscopy